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j hanna 30  (Addgene inc)


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    Structured Review

    Addgene inc j hanna 30
    J Hanna 30, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/j+hanna+30/Nanog-CreER+targeting+construct+(Plasmid+%2359720)/pm37611093-466-7-10
    Average 91 stars, based on 1 article reviews
    j hanna 30 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Construct:

    Article Title: An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogramming.
    Article Snippet: .. Briefly, Nanog-CreERpgk-Hygro targeting construct, a gift from J. Hanna (30) (Addgene, no. 59720) was linearized with Sal1 restriction enzyme (NEB, no. R3138), transfected into the double knock-in mESCs with Lipofectamine Stem Transfection Reagent (Invitrogen, no. STEM00001). .. Twenty-four hours after transfection, the cells were dissociated with 0.05% trypsin into single cells, seeded on Matrigel-coated six-well plates in serum/2iLIF medium containing Hygromycin (100 μg/ml; GoldBio) for 7 to 10 days.

    Transfection:

    Article Title: An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogramming.
    Article Snippet: .. Briefly, Nanog-CreERpgk-Hygro targeting construct, a gift from J. Hanna (30) (Addgene, no. 59720) was linearized with Sal1 restriction enzyme (NEB, no. R3138), transfected into the double knock-in mESCs with Lipofectamine Stem Transfection Reagent (Invitrogen, no. STEM00001). .. Twenty-four hours after transfection, the cells were dissociated with 0.05% trypsin into single cells, seeded on Matrigel-coated six-well plates in serum/2iLIF medium containing Hygromycin (100 μg/ml; GoldBio) for 7 to 10 days.

    Knock-In:

    Article Title: An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogramming.
    Article Snippet: .. Briefly, Nanog-CreERpgk-Hygro targeting construct, a gift from J. Hanna (30) (Addgene, no. 59720) was linearized with Sal1 restriction enzyme (NEB, no. R3138), transfected into the double knock-in mESCs with Lipofectamine Stem Transfection Reagent (Invitrogen, no. STEM00001). .. Twenty-four hours after transfection, the cells were dissociated with 0.05% trypsin into single cells, seeded on Matrigel-coated six-well plates in serum/2iLIF medium containing Hygromycin (100 μg/ml; GoldBio) for 7 to 10 days.



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    j hanna 30  (Addgene inc)
    91
    Addgene inc j hanna 30
    J Hanna 30, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/j+hanna+30/Nanog-CreER+targeting+construct+(Plasmid+%2359720)/pm37611093-466-7-10
    Average 91 stars, based on 1 article reviews
    j hanna 30 - by Bioz Stars, 2026-09
    91/100 stars
    		  Buy from Supplier

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